Molecular Recognition in Biological Systems and Bioinformatics

Immunology; Molecular Biology; Cancer research; Transgenic and Knockout Mice.

My lab is interested in the biological function of the Ets family of transcription factors, in both normal development and in disease. In particular, my recent work has focused on the founding member of the Ets family, Ets-1. Ets-1 is normally expressed at high levels in B and T lymphocytes and we have projects to dissect its function in both of these cell types. In addition, Ets-1 expression is upregulated in a variety of tumors of different organ systems. We have also developed a collaborative project with Dr. Satrajit Sinha to examine the role of Ets-1 in skin cancer.

X-ray film developer

Flow-Jo software for analysis of flow cytometry results

Mouse

Transcription factors (mainly Ets-1, PU.1, Spi-B) also signaling proteins in B cells

Autoimmune diseases like SLE, Cancer, particularly skin cancer

• Flow cytometry
• Adoptive transfer
• ELISA
• ELISPOT
• Immunofluorescence
• Western blot
• Northern blot
• PCR

• A20 B lymphoma cells
• WEHI-231 immature B lymphoma
• P3X plasmacytoma cells
• HeLa
• NIH 3T3
• 293T
• mouse keratinocytes

Systemic lupus erythematosus, cancer

Spleen, thymus, lymph node, skin

http://www.jimmunol.org/cgi/content/full/177/2/905
http://intimm.oxfordjournals.org/cgi/content/abstract/17/9/1179